Figure 6. Effect of GSNO_imm on twitch and tetanic responses.

A, amplitude of twitch and tetanic responses became progressively smaller following a 2 min exposure to 2 mm GSNO_imm, and this was not ameliorated by DTT treatment. Time scale: 1 s for twitches and tetani, 5 s for 15 μm Mg²⁺ and max responses. B, twitch responses to single and paired pulses (as in Fig. 3B), before and after 2 mm GSNO_imm. Control (filled circles), and post-GSNO_imm responses expressed relative to control single pulse response (open circles) or normalized to post-treatment single pulse response (grey squares). Exposure to GSNO_imm did not alter t-system repriming period (∼4 ms).