Table 2.
WT P0–5 | SOD1 P0–5 | WT P6+ | SOD1 P6+ | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Mean | SD n | Mean | SD n | Mean | SD n | Mean | SD n | Significance of age | Significance of SOD1 | |
Vthresh (mV) | −29 | ±5 | −27 | ±5 | −30 | ±7 | −30 | ±5 | — | — |
14 | 16 | 18 | 26 | |||||||
AP overshoot (mV) | 28 | ±7 | 29 | ±10 | 30 | ±11 | 32 | ±7 | — | — |
19 | 19 | 20 | 28 | |||||||
AP duration (ms) | 1.5 | ±0.6 | 1.1 | ±0.3 | 1.1 | ±0.3 | 0.9 | ±0.2 | ↓↓↓ | ↓↓↓ |
19 | 19 | 20 | 28 | |||||||
AP rise (mV ms−1) | 75 | ±26 | 95 | ±33 | 95 | ±31 | 106 | ±20 | ↑↑ | ↑ |
19 | 19 | 20 | 28 | |||||||
AP fall (mV ms−1) | 39 | ±13 | 56 | ±19 | 54 | ±16 | 72 | ±17 | ↑↑↑ | ↑↑↑ |
19 | 19 | 20 | 28 | |||||||
ISI 1–3/SS ISI (%) | 70 | ±34 | 66 | ±22 | 57 | ±32 | 71 | ±25 | — | — |
16 | 15 | 17 | 15 | |||||||
AHP amplitude (mV) | 9.4 | ±2.3 | 8.9 | ±2.3 | 9.2 | ±3.0 | 10.8 | ±2.9 | — | — |
15 | 15 | 11 | 17 | |||||||
AHP τ (ms) | 40 | ±18 | 28 | ±15 | 28 | ±10 | 24 | ±10 | ↓ | ↓ |
15 | 14 | 11 | 17 | |||||||
ADH amplitude (mV) | 5.5 | ±2.7 | 2.9 | ±2.5 | 2.5 | ±1.5 | 1.9 | ±1.6 | ↓↓ | ↓ |
7 | 13 | 13 | 17 | |||||||
Conductance (nS) | 16 | ±6 | 20 | ±10 | 26 | ±10 | 32 | ±14 | ↑↑↑ | ↑ |
24 | 25 | 30 | 32 | |||||||
Ion (pA) | 368 | ±175 | 443 | ±281 | 664 | ±374 | 903 | ±519 | ↑↑↑ | — |
16 | 18 | 18 | 27 | |||||||
Ioff (pA) | 420 | ±201 | 486 | ±293 | 781 | ±425 | 983 | ±583 | ↑↑↑ | — |
16 | 18 | 18 | 27 | |||||||
ΔI (pA) (ms) | 52 | ±134 | 44 | ±86 | 117 | ±195 | 80 | ±243 | — | — |
16 | 18 | 18 | 27 | |||||||
F–Iasc (Hz nA−1) | 35 | ±15 | 40 | ±23 | 31 | ±15 | 33 | ±17 | — | — |
15 | 18 | 17 | 27 | |||||||
F–Idesc (Hz nA−1) | 32 | ±14 | 40 | ±28 | 33 | ±21 | 35 | ±24 | — | — |
13 | 18 | 16 | 27 | |||||||
RMP (mV) | −57 | ±4 | −56 | ±4 | −60 | ±6 | −60 | ±5 | ↓↓↓ | — |
16 | 19 | 20 | 27 |
Shown here are the properties of the action potential (AP), including the voltage at AP threshold (Vthresh), AP overshoot and the interspike intervals (ISIs) of first three APs of a square current pulse normalized to steady state (ISI 1–3/SS ISI). The following rows include conductance, the ascending and descending slope of the F–I curves (F–Iasc, F–Idesc) and the resting membrane potential (RMP). The mean value ± SD for each group is listed with the number of samples (n). The number of arrows in the rightmost two columns indicates significance, as follows: ↑ indicates 0.05 > P≥ 0.01; ↑↑ indicates 0.01 > P≥ 0.001; and ↑↑↑ indicates P < 0.001.