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. 2011 Mar 26;20(12):2333–2343. doi: 10.1093/hmg/ddr126

Figure 7.

Figure 7.

Increased expression of downstream signaling targets of IGF-1 in Lama2Dy-w+IGF-1tg muscle lysates. (A) Western blots of total Akt, phospho-Akt (Thr-308), phospho-Akt (Ser-473) and α-tubulin in muscles isolated from 6-week-old WT, Lama2Dy-w and Lama2Dy-w+IGF-1tg mice. (B) Relative optical density analysis of phospho-Thr-308-Akt levels in Lama2Dy-w+IGF-1tg muscles (black bars) revealed a several-fold increase relative to Lama2Dy-w (white bars) and WT (gray bars), whereas total Akt and phospho-Ser-473-Akt levels increased only marginally. (C) Western blots of phospho-ERK1/2, total ERK1/2 and α-tubulin in 6-week-old muscles isolated from Lama2Dy-w and Lama2Dy-w+IGF-1tg mice. (D) Relative optical density of total ERK1/2 was similar in Lama2Dy-w and Lama2Dy-w+IGF-1tg muscle lysates. However, an increase in relative phospho-ERK1/2 levels was seen in muscles of Lama2Dy-w+IGF-1tg (black bars) compared with its non-transgenic littermates (white bars).