Figure 8.

IGF-1 overexpression leads to a decrease in Lama2^Dy-w-mediated apoptosis. (A) Caspase 3/7 activity was higher in Lama2^Dy-w compared with WT muscles (P< 0.0001). There was only a 12.8% decrease in caspase activity in the muscle lysates of Lama2^Dy-w+IGF-1tg mice compared with its non-transgenic littermates (n= 5, P< 0.05). (B) Representative muscle sections of Lama2^Dy-w (top panel) and Lama2^Dy-w+IGF-1tg (bottom panel) assayed for TUNEL-positive nuclei did not show any significant difference. TUNEL-positive nuclei (green) are indicated by white arrows. These sections were also stained with PAN laminin isoform (red) and DAPI (blue).