Figure 5.

Adenine ameliorated altered Hdh^Q111/Q111 primary striatal neuron neuritogenesis. Confocal images of primary Hdh^Q111/Q111 striatal cell cultures at 10 days (div10), after culture in media supplemented with vehicle (DMSO) or with adenine (Adenine), showing MAP2-immunostained microtubules (red) and DAPI-stained nuclei (blue) and overlapped images (merge), illustrating that adenine-treated neuronal cells displayed a less branched neurite network than vehicle-treated cells. The bar graph below shows that adenine supplementation significantly decreased the total length of neurites/cell by 25% (*P = 0.001), while a similar number of DAPI-stained nuclei confirmed that adenine did not result in toxicity or loss of cells. Error bars represent standard errors.