Table 1.
iTRAQ identification of proteins with >20% increased expression and peptide count >1 in the unlesioned sciatic nerve from ApoE4 mice compared with ApoE3 mice
| Protein name | Accession number | Protein MW | Protein PI | Peptide count | Total ion score, CI percentage | Best ion score | Average ratio (E4/E3) | Standard deviation (E4/E3) |
|---|---|---|---|---|---|---|---|---|
| Haptoglobin precursor | 8850219 | 42455.7 | 5.9 | 7 | 100.00 | 67.94 | 3.39 | 1.42 |
| Orosomucoid 1 | 15215270 | 26393.8 | 5.4 | 5 | 100.00 | 58.65 | 2.05 | 0.53 |
| Myomesin 2 | 170763465 | 179757.5 | 5.6 | 11 | 100.00 | 72.44 | 1.80 | 0.35 |
| PK-120 precursor | 2739028 | 112522.4 | 6.1 | 2 | 100.00 | 52.16 | 1.67 | 0.03 |
| Myosin, heavy polypeptide 4, skeletal muscle | 67189167 | 254149.5 | 5.6 | 85 | 100.00 | 127.38 | 1.67 | 0.44 |
| Haemopexin precursor | 160358829 | 55053.3 | 7.9 | 15 | 100.00 | 95.47 | 1.63 | 0.42 |
| Muscle glycogen phosphorylase | 6755256 | 104509.5 | 6.7 | 18 | 100.00 | 93.56 | 1.63 | 0.33 |
| Myosin-binding protein C, fast type | 81910387 | 143381.3 | 6.0 | 10 | 100.00 | 100.37 | 1.62 | 0.49 |
| ATPase, Ca++ transporting, ubiquitous | 74215005 | 116671.4 | 5.5 | 2 | 100.00 | 48.16 | 1.61 | 0.09 |
| Major urinary protein | 295910 | 22495.3 | 4.9 | 3 | 100.00 | 85.86 | 1.59 | 0.23 |
| Major urinary protein | 13276755 | 22609.4 | 5.0 | 3 | 100.00 | 85.86 | 1.58 | 0.21 |
| MUP | 755765 | 17535.8 | 4.9 | 2 | 100.00 | 62.95 | 1.53 | 0.12 |
| Myh7 protein | 187956918 | 252607.1 | 5.6 | 20 | 100.00 | 91.72 | 1.51 | 0.56 |
| Alpha cardiac myosin heavy chain | 191618 | 253883.5 | 5.6 | 18 | 100.00 | 91.72 | 1.48 | 0.58 |
| Muscle creatine kinase | 6671762 | 48101.2 | 6.6 | 14 | 100.00 | 101.99 | 1.45 | 0.34 |
| S100 calcium binding protein A9 (calgranulin B) | 6677837 | 14907.5 | 6.6 | 2 | 100.00 | 69.01 | 1.45 | 0.09 |
| Solute carrier family 4 (anion exchanger), member 1 | 74138529 | 108215.2 | 5.4 | 2 | 100.00 | 50.97 | 1.45 | 0.01 |
| Fibrinogen, gamma polypeptide | 74143561 | 55480.4 | 5.4 | 6 | 100.00 | 84.66 | 1.43 | 0.09 |
| Titin | 123232572 | 3312853.3 | 6.2 | 2 | 100.00 | 37.84 | 1.43 | 0.05 |
| Fibrinogen beta chain precursor | 33859809 | 60169.0 | 6.7 | 12 | 100.00 | 78.56 | 1.41 | 0.29 |
| Myosin, heavy polypeptide 8, skeletal muscle, perinatal | 71143152 | 253848.8 | 5.7 | 46 | 100.00 | 106.43 | 1.40 | 0.58 |
| Parvalbumin | 31980767 | 14372.8 | 5.0 | 5 | 100.00 | 88.79 | 1.40 | 0.19 |
| Myosin, heavy polypeptide 8, skeletal muscle, perinatal | 187956525 | 253835.8 | 5.7 | 46 | 100.00 | 106.43 | 1.39 | 0.57 |
| Haemoglobin, beta adult major chain | 31982300 | 17513.4 | 7.1 | 13 | 100.00 | 107.43 | 1.36 | 0.22 |
| Clusterin precursor | 214010170 | 56451.5 | 5.5 | 4 | 100.00 | 82.73 | 1.36 | 0.47 |
| mCG18455 | 148678485 | 256334.6 | 5.5 | 22 | 100.00 | 84.80 | 1.36 | 0.71 |
| Troponin T | 2340048 | 34082.0 | 9.4 | 4 | 100.00 | 78.26 | 1.35 | 0.16 |
| Haemoglobin alpha, adult chain 2 | 145301549 | 16878.0 | 8.0 | 9 | 100.00 | 101.60 | 1.35 | 0.25 |
| ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 | 148685412 | 116243.4 | 5.3 | 12 | 100.00 | 114.25 | 1.35 | 0.24 |
| Actinin alpha 3 | 7304855 | 110400.7 | 5.3 | 34 | 100.00 | 112.37 | 1.34 | 0.46 |
| Phosphofructokinase, muscle | 13529638 | 92278.8 | 8.2 | 8 | 100.00 | 67.00 | 1.34 | 0.11 |
| Collagen, type VI, alpha 1 precursor | 6753484 | 117555.5 | 5.2 | 7 | 100.00 | 91.16 | 1.33 | 0.17 |
| Glutamine synthetase | 31982332 | 45842.4 | 6.6 | 2 | 100.00 | 67.62 | 1.33 | 0.04 |
| Myomesin 1 isoform 2 | 145279198 | 194032.5 | 6.0 | 7 | 100.00 | 81.84 | 1.33 | 0.13 |
| Sarcalumenin, isoform CRA_a | 148664814 | 59761.7 | 6.2 | 4 | 100.00 | 60.15 | 1.31 | 0.25 |
| ATPase, Ca++ transporting, slow twitch 2 isoform a | 158635979 | 124907.5 | 5.2 | 5 | 100.00 | 68.51 | 1.31 | 0.33 |
| Histone cluster 1, H1c | 9845257 | 29755.8 | 11.0 | 2 | 100.00 | 75.68 | 1.30 | 0.17 |
| mCG2006, isoform CRA_a | 148680420 | 187536.8 | 6.3 | 2 | 100.00 | 60.73 | 1.30 | 0.12 |
| Glycogen phosphorylase, liver form | 20178036 | 107417.7 | 6.6 | 5 | 100.00 | 58.28 | 1.29 | 0.20 |
| Phosphoglycerate mutase 2 | 9256624 | 31972.9 | 8.7 | 8 | 100.00 | 89.17 | 1.29 | 0.17 |
| Enolase 3, beta muscle, isoform CRA_a | 148680653 | 54402.9 | 6.8 | 15 | 100.00 | 112.63 | 1.28 | 0.28 |
| Sorbitol dehydrogenase | 22128627 | 42431.2 | 6.6 | 2 | 100.00 | 54.45 | 1.28 | 0.23 |
| RIKEN cDNA 1810020D17, isoform CRA_c | 148684357 | 17880.5 | 7.8 | 2 | 100.00 | 44.25 | 1.27 | 0.04 |
| Unnamed protein product | 26345578 | 19080.7 | 10.5 | 3 | 100.00 | 67.62 | 1.26 | 0.17 |
| Cullin 1 | 7549752 | 100115.9 | 8.2 | 2 | 100.00 | 53.35 | 1.26 | 0.23 |
| Hormone-sensitive lipase (Mus musculus domesticus) | 677885 | 87018.7 | 6.5 | 2 | 100.00 | 60.78 | 1.25 | 0.03 |
| Epoxide hydrolase 1 | 6753762 | 57724.5 | 8.4 | 2 | 100.00 | 53.93 | 1.24 | 0.10 |
| Collagen, type VI, alpha 2 precursor | 22203747 | 118822.8 | 6.0 | 11 | 100.00 | 63.77 | 1.24 | 0.16 |
| Aldolase A, fructose-bisphosphate | 42490830 | 43401.8 | 8.5 | 11 | 100.00 | 91.23 | 1.23 | 0.10 |
| Preprocomplement component C3 | 309122 | 205041.7 | 6.4 | 6 | 100.00 | 56.08 | 1.23 | 0.18 |
| Phosphatidylinositol-4-phosphate 5-kinase type II beta | 17223780 | 50243.1 | 6.4 | 2 | 100.00 | 52.71 | 1.23 | 0.27 |
| Fast skeletal myosin alkali light chain 1 isoform 1f | 29789016 | 23699.5 | 5.0 | 14 | 100.00 | 97.84 | 1.22 | 0.18 |
| Adenylate kinase 1 | 10946936 | 26311.9 | 5.7 | 5 | 100.00 | 77.64 | 1.22 | 0.24 |
| Fibrinogen, alpha polypeptide, isoform CRA_b | 148683477 | 68946.6 | 7.0 | 6 | 100.00 | 64.58 | 1.21 | 0.23 |
| Lactate dehydrogenase A isoform 1 | 6754524 | 40772.0 | 7.6 | 13 | 100.00 | 74.65 | 1.21 | 0.13 |
| Chain B, X-ray structure of the nucleosome core particle | 27573730 | 12958.6 | 11.4 | 4 | 100.00 | 53.05 | 1.21 | 0.14 |
| 2′,3′-cyclic nucleotide 3′ phosphodiesterase isoform 2 | 226423907 | 53755.9 | 9.1 | 24 | 100.00 | 122.44 | 1.21 | 0.17 |
| ATPase, H+ transporting, lysosomal V1 subunit H | 14318722 | 60928.4 | 6.2 | 2 | 100.00 | 61.01 | 1.20 | 0.02 |
| Chaperonin | 460317 | 64225.7 | 8.2 | 2 | 100.00 | 98.95 | 1.20 | 0.15 |
| Tyrosyl-tRNA synthetase | 165377181 | 68152.2 | 6.6 | 2 | 100.00 | 40.36 | 1.20 | 0.14 |
| Glyoxalase 1 | 19354350 | 23702.3 | 5.2 | 2 | 100.00 | 47.51 | 1.20 | 0.33 |
| Xanthine dehydrogenase | 187954915 | 159925.8 | 7.6 | 3 | 100.00 | 51.62 | 1.20 | 0.06 |