Table 3.
iTRAQ identification of proteins with >20% increased expression and peptide count >1 in regenerating sciatic nerve from ApoE4 mice compared with ApoE3 mice
| Protein name | Accession number | Protein MW | Protein PI | Peptide count | Total ion score, CI percentage | Best ion score | Average ratio (E4/E3) | Standard deviation (E4/E3) |
|---|---|---|---|---|---|---|---|---|
| Haptoglobin precursor | 8850219 | 42455.7 | 5.9 | 7 | 100.00 | 67.94 | 3.33 | 0.12 |
| Orosomucoid 1 | 15215270 | 26393.8 | 5.4 | 5 | 100.00 | 58.65 | 1.90 | 0.15 |
| PK-120 precursor | 2739028 | 112522.4 | 6.1 | 2 | 100.00 | 52.16 | 1.87 | 0.01 |
| Haemopexin precursor | 160358829 | 55053.3 | 7.9 | 15 | 100.00 | 95.47 | 1.51 | 0.12 |
| Calpain small subunit | 19705421 | 24752.3 | 5.1 | 3 | 100.00 | 41.83 | 1.42 | 0.13 |
| Unnamed protein product | 26345578 | 19080.7 | 10.5 | 3 | 100.00 | 67.62 | 1.41 | 0.17 |
| Laminin B1 subunit 1 | 114326497 | 221889.2 | 4.9 | 13 | 100.00 | 77.25 | 1.39 | 0.13 |
| Fibrinogen, alpha polypeptide, isoform CRA_b | 148683477 | 68946.6 | 7.0 | 6 | 100.00 | 64.58 | 1.38 | 0.09 |
| Fibrinogen, gamma polypeptide | 148683478 | 55480.4 | 5.4 | 6 | 100.00 | 84.66 | 1.38 | 0.09 |
| CD10 neutral endopeptidase 24.11 | 192459 | 93776.9 | 5.7 | 2 | 100.00 | 52.20 | 1.37 | 0.23 |
| Fibrinogen beta chain precursor | 33859809 | 60169.0 | 6.7 | 12 | 100.00 | 78.56 | 1.35 | 0.12 |
| C4 complement protein | 50242 | 137077.1 | 6.5 | 2 | 100.00 | 47.28 | 1.34 | 0.13 |
| 2′,3′-cyclic nucleotide 3′ phosphodiesterase isoform 2 | 226423907 | 53755.9 | 9.1 | 24 | 100.00 | 122.44 | 1.33 | 0.13 |
| Aspartyl-tRNA synthetase isoform 1 | 211065507 | 61980.2 | 6.1 | 2 | 100.00 | 63.91 | 1.33 | 0.03 |
| Predicted: similar to major urinary protein 1 | 149252557 | 28801.5 | 5.1 | 4 | 100.00 | 85.86 | 1.32 | 0.08 |
| Predicted: hypothetical protein isoform 2 | 82886943 | 18543.3 | 10.3 | 3 | 100.00 | 58.44 | 1.31 | 0.12 |
| Hypoxanthine-guanine phosphoribosyltransferase | 2499938 | 26555.9 | 5.7 | 3 | 100.00 | 48.91 | 1.30 | 0.08 |
| Neural cell adhesion molecule 1 isoform 1 | 124517689 | 101275.8 | 4.8 | 3 | 100.00 | 46.03 | 1.29 | 0.13 |
| Glutamine synthetase | 31982332 | 45842.4 | 6.6 | 2 | 100.00 | 67.62 | 1.28 | 0.05 |
| Nucleoside phosphorylase | 7305395 | 34057.1 | 5.8 | 3 | 100.00 | 67.49 | 1.28 | 0.06 |
| Auh protein | 20072952 | 36020.8 | 9.6 | 2 | 100.00 | 56.44 | 1.27 | 0.04 |
| Preprocomplement component C3 | 309122 | 205041.7 | 6.4 | 6 | 100.00 | 56.08 | 1.27 | 0.15 |
| Microtubule-associated protein 6 isoform 1 | 113204613 | 107716.5 | 9.5 | 5 | 100.00 | 70.91 | 1.27 | 0.10 |
| Nidogen 1 precursor | 171543883 | 143072.8 | 5.2 | 23 | 100.00 | 76.48 | 1.27 | 0.10 |
| Fbxo2 protein | 20072543 | 34278.6 | 4.3 | 6 | 100.00 | 82.62 | 1.27 | 0.17 |
| Laminin, alpha 2 | 117647249 | 376116.8 | 5.8 | 26 | 100.00 | 107.74 | 1.26 | 0.15 |
| Breast carcinoma amplified sequence 1 | 123233481 | 58733.9 | 6.2 | 4 | 100.00 | 83.08 | 1.26 | 0.09 |
| Solute carrier family 44, member 2 | 22779895 | 86584.2 | 9.1 | 2 | 100.00 | 66.29 | 1.25 | 0.15 |