Skip to main content
. 2011 Apr 12;20(12):2322–2332. doi: 10.1093/hmg/ddr125

Figure 6.

Figure 6.

PNKD-L does not rescue lost HAGH activity. (A) Protein extracts from a Drosophila HAGH homozygous null mutant (circles) lose the ability to hydrolyze SLG when compared with protein extracts from WT flies (triangles). When Drosophila HAGH is replaced in the null mutant background by transgenic over-expression (squares), SLG hydrolytic activity is restored to levels even greater than detected in WT flies (triangles). However, when human PNKD-L is expressed on a Drosophila HAGH mutant background, no rescue of SLG hydrolysis is detected (x's). (B) Mut-Tg mice and KO mice have lower glutathione levels in extracts from the cortex compared with WT littermates under normal conditions. Error bars indicate standard errors (WT, n = 13; mut-Tg, n = 12; KO, n= 8). *P < 0.05 and **P < 0.01 from WT mice (Student's t-test).