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. Author manuscript; available in PMC: 2011 May 20.
Published in final edited form as: Stem Cell Stud. 2010 Nov 23;1(1):e5. doi: 10.4081/scs.2010.e5

Figure 4.

Figure 4

Improved detection of human cells in mouse bone marrow using doublet discrimination. A live cell gate was used to eliminate dead cells stained with PI as shown (top-left). Untransplanted NSG-BM (center column) and a mouse transplanted with human FBM 229 days prior (right column) were analyzed using only live cell gating (top row) or live cell gating and doublet discrimination (center row). The staining pattern of doublet mouse and/or human cells, excluded by the doublet discrimination, is shown in the bottom row. Numbers represent the percentage of cells that fall within the gated regions.