Figure 5. Quantification of melanocyte phenotype at 5 dpf in embryos treated with ethanol at different stages.

‘Contracted morphology’ indicates that the cell is rounded, and the melanosomes concentrated into a small area (Figure 4 A). ‘Dispersed’ morphology (Figure 4 B,C) indicates that the yolk sac melanocytes were squamate and the melanosomes distributed across a wider area than in Figure 4A. As can be seen in the graph, the dispersed morphology is characteristic of ethanol-treated embryos, and reaches a maximum in embryos treated at prim-16. Italic numbers = N embryos.