TABLE 3.
Enumeration of V. vulnificus from inoculated oysters
| Inoculum (log CFU ml−1) | Result for:
|
|
|---|---|---|
| DNA probea (log CFU ml−1 ± SD) | Real-time PCRb (log CFU ml−1 ± SD) (Ct ± SD) | |
| 7.3 | 7.0 ± 0.01 | 7.7 ± 0.02 (19.5 ± 0.08) |
| 6.3 | 5.8 ± 0.14 | 6.8 ± 0.05 (22.7 ± 0.17) |
| 5.3 | 4.9 ± 0.11 | 5.7 ± 0.02 (26.9 ± 0.06) |
| 4.3 | 4.0 ± 0.07 | 4.8 ± 0.01 (30.3 ± 0.06) |
| 3.3 | 3.7 ± 0.06 | 4.0 ± 0.03 (33.3 ± 0.10) |
| 2.3 | 3.6 ± 0.17 | 3.6 ± 0.04 (34.8 ± 0.15) |
a
V. vulnificus MO6-24/O levels in inoculated oyster homogenate were determined by colony blot hybridization using the VVAP alkaline phosphatase probe (36) as described in the text.
b
Real-time PCR determination of V. vulnificus concentrations was based on the mean of triplicate samples. Concentrations were derived from a standard curve by using the mean of triplicate Ct values for serial 10-fold dilutions of DNA extracted from known concentrations of bacteria as described in text.