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. 2011 May 20;6(5):e19825. doi: 10.1371/journal.pone.0019825

Figure 2. Screening of anti-influenza phytochemicals using a PA endonuclease-inhibition assay.

Figure 2

Screening of phytochemicals for anti-influenza A activity using a PA endonuclease assay. The effects of the various phytochemicals upon the endonuclease activity of the PA N-terminal domain of the influenza A RNA polymerase were tested. The recombinant PA N-terminal domain protein was added to each reaction at 0.35 µg/ 100 µl. A zero control (no PA domain added) was also assayed. Phytochemicals were added at a 1 or 10 µM dose and M13mp18 was used as the substrate.