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. 2011 May 20;6(5):e19958. doi: 10.1371/journal.pone.0019958

Figure 5. Intra-LA infusion of a DNMT inhibitor impairs auditory fear memory consolidation.

Figure 5

(A) Schematic of behavioral protocol. Rats were conditioned with 3 tone-shock pairings, followed by intra-LA infusion of vehicle or 5-AZA 1 hr later (n = 5, each group). LTM was assessed ∼24 hr after training in a distinct context. Rats were re-conditioned drug free and re-tested for LTM ∼1 week later. (B) Mean (±SEM) post-shock freezing scores in each group following each conditioning trial. (C) Mean (±SEM) LTM retention test scores across each trial. (D) Mean (±SEM) LTM retention re-test scores across each trial following re-conditioning one week later. (E) Schematic of behavioral protocol. Rats were conditioned with 3 tone-shock pairings, followed 1 hr later by intra-LA infusion of vehicle (n = 4) or 5-AZA (n = 4). STM was assessed 1 hr after training in a distinct context. (F) Mean (±SEM) post-shock freezing scores in each group following each conditioning trial. (G) Mean (±SEM) STM retention test scores across each trial. (H) Histological verification of cannula placements for rats in LTM (left) and STM (right) experiments infused with vehicle (black circles) or 5-AZA (white circles). Panels adapted from Paxinos and Watson [40].