Figure 3. Effect of intraperitoneal (ip) administration of saline solution alone or containing AICAR (1.2 g·kg−1) or metformin (120 mg·kg−1) on rainbow trout liver ACC phosphorylation status (at serine 79; Western blot analysis).
Fish were fasted (48 h) prior to injection and livers were sampled 2 h after injection. Gels were loaded with 20 µg total protein per lane. Protein and phosphorylation levels were normalized to tissue β-tubulin levels. Results are expressed as means ± s.e.m. (n = 6) and were analyzed by one-way ANOVA followed by Student-Newman-Keuls comparison test. *Significant difference (α<0.05).