. 2011 May 20;6(5):e20228. doi: 10.1371/journal.pone.0020228

Table 2. In vivo (2 h after ip administration) and in vitro (16 h of incubation) effects of AICAR (1.2 g·kg⁻¹ or 0.5 mM in the medium) or metformin (120 mg·kg⁻¹ or 0.5 mM) in rainbow trout on mRNA transcript levels of hepatic genes representative of glucose and lipid metabolism.

	In vivo			In vitro
	Control	AICAR	Metformin	Control	AICAR	Metformin
GK	1.05±0.2	0.32±0.06^*	18.48±2.03^*	1.06±0.2	0.32±0.05^*	0.89±0.12
G6Pase	1.12±0.11	0.51±0.05^*	1.02±0.09	1.52±0.71	0.89±0.38^*	1.38±0.49
FBPase	1.10±0.18	1.50±0.19	1.43±0.21	1.03±0.16	0.90±0.12	1.08±0.13
PEPCK	1.16±0.19	1.17±0.23	0.53±0.11	1.03±0.15	1.02±0.18	1.00±0.20
FAS	1.09±0.16	2.51±0.21^*	0.67±0.17	1.05±0.19	0.29±0.05^*	096±0.16

GK (glucokinase), G6Pase (glucose 6-phosphatase), FBPase (fructose 1,6-bisphosphatase), PEPCK (phosphoenolpyruvate carboxykinase), FAS (fatty acid synthase) Results are expressed as means ± s.e.m. (n = 6) and were analyzed by one-way ANOVA followed by Student–Newman–Keuls comparison test.

*Significant difference (P<0.05). mRNA levels were estimated using real-time RT-PCR and normalized to elongation factor 1α (EF1α) transcripts which did not change under the experimental conditions.

Data are presented as relative ratios of the target gene to the reference gene (EF1α).

Table 2. In vivo (2 h after ip administration) and in vitro (16 h of incubation) effects of AICAR (1.2 g·kg−1 or 0.5 mM in the medium) or metformin (120 mg·kg−1 or 0.5 mM) in rainbow trout on mRNA transcript levels of hepatic genes representative of glucose and lipid metabolism.

Table 2. In vivo (2 h after ip administration) and in vitro (16 h of incubation) effects of AICAR (1.2 g·kg⁻¹ or 0.5 mM in the medium) or metformin (120 mg·kg⁻¹ or 0.5 mM) in rainbow trout on mRNA transcript levels of hepatic genes representative of glucose and lipid metabolism.