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. 2011 May 20;6(5):e20154. doi: 10.1371/journal.pone.0020154

Figure 3. Demethylation is dependent on DNA replication.

Figure 3

(A) Detection of DNA replication at various times in one-cell SCNT embryos by BrdU labeling. (Left) At 6 h post-activation, a few embryos were labeled by BrdU and were a dark color, indicating the beginning of DNA replication. (Middle) At 12 h post-activation, most embryos were labeled by BrdU and were a bright color, indicating the peak of DNA replication. (Right) At 15 h post-activation, few embryos were labeled by BrdU and were dark in color again, indicating near completion of DNA replication. (B) Methylation status of DMRs at 6 and 15 h in one-cell SCNT embryos. (Left) At 6 h post-activation, methylation was unchanged compared with donor cells. (Right) At 15 h post-activation, demethylation had occurred, and each DMR lost about one-quarter of its normal methylation. (Lar) Large White-derived allele; (Nor) Northeast Min-derived allele.