Figure 5. Myelin protein levels are reduced in aspa^lacZ/lacZ mutants.

Western blot analysis of PLP, DM20, CNP and MBP levels in whole brain lysates of aspa^+/+, aspa^lacZ/+ and aspa^lacZ/lacZ mice (P60, n = 3). Quantification of immunoblots shows statistically significant decreases of all proteins tested in homozygous mutants while the presence of one functional aspa allele is sufficient to preserve protein quantities at control levels. Note: PLP, DM20, and the MBP21.5 kD and MBP18.5/17 kD isoforms were analysed separately.