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. Author manuscript; available in PMC: 2011 Jul 29.
Published in final edited form as: Neuron. 2010 Jul 29;67(2):199–212. doi: 10.1016/j.neuron.2010.06.021

Figure 3.

Figure 3

Knockdown of MMP-10 and MMP-14 and inhibitors of MMP activity block caspase activation in striatal Hdh111Q/111Q cells. (A) Quantitative RT-PCR validation of siRNA knockdown. t-test was performed (p = 0.0004 and p = 0.7E-9, respectively) (B) Western blot analysis of siRNA targeting on the expression of the indicated MMP. (C) Effect of siRNA to MMP-10, MMP-14 or non-targeting on activation of caspase-3 in Hdh111Q/111Q cells during 24 h serum withdrawal. Caspase activity is in units dRFU/min/mg (ANOVA, n= 5, *p < 0.05 ***, p < 0.001). (D) Inhibition of MMP’s with NNGH lowers caspase activation in striatal cells (ANOVA analysis, p = 0.027 and 0.005). (E) TIMP1 and TIMP3 overexpression of protein reduces caspase activation in HD striatal cells ( t-test was performed, p = 0.3E-6 and 0.007).