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. Author manuscript; available in PMC: 2012 Jun 15.
Published in final edited form as: Dev Biol. 2011 Apr 13;354(2):242–252. doi: 10.1016/j.ydbio.2011.04.002

Fig. 6.

Fig. 6

Loss of Nfib in mesenchyme increases TTF-1 positive and TTF-1 negative cell proliferation. Paraffin sections of E18.5 and E16.5 lungs from Nfibflox/flox and Nfibflox/flox, D1-Cre embryos were stained for both Ki67 and TTF-1 (A, B, C, D). Insets show a 5X magnification. Immunofluorescence staining reveals there were more Ki67+TTF-1+ cells (arrows) in Nfibflox/flox, D1-Cre lungs compared with Nfibflox/flox lungs at E16.5 and E18.5 (E). The percentage of Ki67+TTF-1− cells was also higher in Nfibflox/flox, D1-Cre lungs at E16.5 (F). In contrast, the percentage of Ki67+TTF-1− cells was similar between Nfibflox/flox and Nfibflox/flox, D1-Cre lungs at E18.5 (F). Arrows denote the colocalization of Ki67 and TTF-1 and the region of magnification; *P<0.05; Scale bar, 50 µm.