Fig. 5.

TLR ligands induce PGE₂ production by DRGNs. Supernatants of DRGNs cultures were collected and PGE₂ was detected using EIA following stimulation by poly I:C (25 μg/ml), gardiquimod (3 μg/ml) or ODN 1826 (32 μg/ml) for 16 hrs. Each bar represents the mean ± S.E.M. (n=10). (A) PGE₂ production by DRGNs from Swiss embryonic mice, and (B) PGE₂ production by DRGNs from C57 and MyD88 knock out embryonic mice. **P <0.01, ***P <0.001 compared with corresponding control, ^##P <0.01 compared with corresponding C57 mice treated with gardiquimod and ODN 1826. Statistical analysis was performed using Student’s t test for two-group comparisons.