Fig. 7.

TRPV1 is expressed by DRGNs and relocates following TLR ligands stimulation. Confocal images of TRPV1 expressed by primary cultured mouse DRGNs are shown. DRGNs were stained with TRPV1 (green), neuron-specific beta-III tubulin (red) and DAPI (blue). Shown are monochrome images of the green fluorescence channel, and merged color images from all channels. (A) control, (B) poly I:C (25 μg/ml), (C) gardiquimod (3 μg/ml), and (D) ODN 1826 (32 μg/ml) stimulation for 16 hrs induced TRPV1 expression in DRGNs. Scale bars: 50 μm. (E) The average intensity of TRPV1 in the neuron surface and dendrites. Images intensity analysis was performed using ImageJ software. Statistical analysis was performed using Student’s t test for two-group comparisons. **P < 0.01 compared with control.