Table 1.

(A) Fold changes in cytokines and chemokines mRNA levels and (B) protein concentrations after stimulated of DRGNs with TLRs ligands.

	Ligand	Cytokines and Chemokines
		CCL5	CXCL10	IL1-alpha	IL1-beta
(A) mRNA fold increased compared to Control	polyI:C (25 μg/ml)	289.9±7.4*	73.8±5.4*	9.7±1.9*	10.9±1.9*
	Gardiquimod (3 μg/ml)	16.9±1.56*	7.4±0.35*	8.9±0.69*	10.4±1.7*
	ODN1826 (32 μg/ml)	9.1±0.69*	7.6±0.17*	6.4±1.7*	6.6±1.4*
(B) Protein concentration (pg/ml)	Control	16.6±2.9	193.9±58.3	0.6±0.17	1.2±0.34
	polyI:C (25 μg/ml)	1899.6±762.6*	26455.8±9714.8*	2.3±1.2*	7.8±2.9*
	Gardiquimod (3 μg/ml)	76.8±25.8	505.7±225.1	3.2±0.17*	2.8±0.52*
	ODN1826 (32 μg/ml)	25.0±5.2	410.0±206.0	1.6±0.34*	3.3±0.69*

DRG cultures were treated with TLRs ligands for 16 hrs. Total RNA was isolated from neurons by RNA isolation kit and reverse-transcribed into cDNA. Each PCR array for quantitative PCR reaction was carried out. The protein levels in supernatants of DRG cultures were determined by multiplex array. The results represent the mean ± SD Data for RNA array were analyzed by RT² Profiler PCR Array Data Analysis software (SABioscience). Statistical analysis for the protein levels were performed by Student’s t test.

^*P<0.05 compared with control n = 3.