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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: J Immunol. 2011 May 2;186(11):6091–6095. doi: 10.4049/jimmunol.1100427

Figure 2. IL-17 production by dermal γδ T cells but not DETCs.

Figure 2

(A) IL-17A staining of digested epidermal and dermal sheets, stimulated with PMA/I for 2 h, gated on total TCRγδ+ cells. Histrogram plot shows RORγt-GFP reporter expression by the indicated cells. (B) IL-17A, IL-17F, and IL-22 mRNA quantification by RT-PCR in sorted dermal CCR6+TCRγδint T cells and total dermal and epidermal sheets. (C) IL-17A staining of PLN and total skin cells stimulated for 8 h with IL-1β/IL-23 or media alone, gated on either CCR6+TCRγδint cells (dermis), CCR6-TCRγδhi cells (epidermis), or CCR6+TCRγδ+ (PLN). Gray lines indicate unstained controls. Data are representative of at least two experiments.