Figure 3. Reconstitution of dermal and PLN TCRγδint CCR6hi T cells by transfer of neonatal thymocytes.
(A–C) γδT cell frequency and IL-17A expression following stimulation with PMA/I for 2 h of (A) PLN and dermal sheet cells from an irradiated mouse reconstituted with BM cells, (B) neonatal (D0-2) or adult thymocytes, and (C) PLN and dermal sheet cells from an irradiated mouse reconstituted with neonatal thymocytes and BM cells. Left panels are gated on CD45+ cells; numbers indicate fraction of total cells. Data are representative of at least three experiments.
