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Immunoglobulin profiles in outbred CF-1 mice immunized on Day 0 with 50 µg/animal of α-lactalbumin, or α-lactalbumin covalently coupled with 5 equivalents of 2, or α-lactalbumin mixed with 5 equivalents of 1. Animals (5 per cohort) were boosted once on Day 14 exactly as mentioned above, and bled on Day 21. α-lactalbumin-specific immunoglobulin levels were quantified by standard antibody-capture ELISA, performed in liquid handler-assisted 384-well format.
