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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Immunol. 2011 Apr 27;186(11):6597–6606. doi: 10.4049/jimmunol.1004074

FIGURE 1.

FIGURE 1

Stat4/Stat6/T-bet TKO have Th17 but not Th1 or Th2 responses. A and B, Stat4 deficiency abolished IL-12–mediated IFN-γ production. Wild-type, Stat6/T-bet DKO, and Stat4/Stat6/T-bet TKO CD4+CD25− T cells were stimulated with plate-bound anti-CD3 (5 µg/ml) and soluble anti-CD28 (1 µg/ml), with or without IL-12 (10 ng/ml). Quantitative RT-PCR for IFN-γ expression was performed on day 2; ELISA for IFN-γ and intracellular staining for IFN-γ and IL-4 were performed on day 3. **p < 0.01, p < 0.001. C, Stat4 deficiency did not alter Th2 differentiation. Intracellular staining for IL-13 in CD4+CD25− T cells after anti-CD3 plus anti-CD28 stimulation, with or without IL-4 (20 ng/ml) for 3 d. D, Stat4 deficiency did not alter composition of IL-10 expression in Th17 cells. Intracellular IL-10 and IL-17 staining in CD4+CD25− T cells after 3 d of stimulation with anti-CD3 and anti-CD28 combined with IL-6 (10 ng/ml) or IL-6 plus TGF-β (5 ng/ml).