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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Immunol. 2011 Apr 27;186(11):6597–6606. doi: 10.4049/jimmunol.1004074

FIGURE 3.

FIGURE 3

Neither Stat4 nor T-bet is required for Th1 development. A, Cell distribution, migration, and proliferation after adoptive transfer. eFluor 670-labeled CD4+CD45RBhigh cells from DKO or TKO mice were injected into SCID mice and harvested 5 or 15 d later, and proliferation analysis was performed on gated CD4 cells from MLN, spleen, and thymus. B, Distribution of eFluor 670-labeled CD4+CD45RBhigh cells in MLN, spleen and thymus 5 d after T cell reconstitution (original magnification ×200). Representative sections from five mice in each group are shown. C and D, Quantitative RT-PCR for IFN-γ and Eomes expression in MLN CD4 T cells purified 8 wk after adoptive transfer. **p < 0.01.