FIGURE 5.
Stat4 deficiency results in higher levels of Foxp3+ Tregs. A, Immunohistochemistry for CD4 and Foxp3 in the distal colons and MLN in SCID mice 8 wk after T cell reconstitution (original magnification ×200). Representative sections are shown from 10 mice in each group. B, Intracellular staining for Foxp3 on gated MLN CD4 cells 8 wk after adoptive transfer (upper panels) and real-time RT-PCR for Foxp3 expression on MLN CD4 T cells 2 or 8 wk after adoptive transfer (lower panel). **p < 0.01.