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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Immunol. 2011 Apr 27;186(11):6597–6606. doi: 10.4049/jimmunol.1004074

FIGURE 6.

FIGURE 6

IL-12/Stat4 signals alter the balance between Th17 and Tregs. A, IL-12/Stat4 suppresses Foxp3 expression. Wild-type, DKO, and TKO CD4+CD25− T cells were stimulated with anti-CD3 and anti-CD28 in the presence of low-dose IFN-γ (1 ng/ml) for 3 d. Intracellular staining for Foxp3 was performed under Treg conditions (TGF-β), with or without IL-12, IL-6, IL-12 plus IL-6, or IL-23 plus IL-6. B, IL-12 does not affect Th17 development. Intracellular IL-17 and IFN-γ expression were measured under Th17 conditions (IL-6 + TGF-β), with or without IL-12 or IL-23. Data are representative of three independent experiments with similar results.