Stat4 inhibits Tregs while promoting Th17 development driven by CD103− CD11chigh MLN DCs. CD4+CD25− T cells from DKO and TKO mice were cultured with wild-type CD103− or CD103+ MLN CD11chigh DCs in the presence of anti-CD3 (1 µg/ml) and IL-2 (1 ng/ml) for 3 d. A, Intracellular staining for Foxp3, IL-17, and IFN-γ expression was performed and analyzed on gated CD4 cells. B, The amounts of IL-12 p70 and IL-27 in the culture supernatants were measured by ELISA. Data are representative of three independent experiments with similar results. nd, not detected.