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. Author manuscript; available in PMC: 2012 May 20.
Published in final edited form as: ACS Chem Biol. 2011 Feb 16;6(5):432–443. doi: 10.1021/cb100253e

Figure 1. Structure of Stat3 inhibitor compounds and effect of C48 on STAT DNA-binding activity.

Figure 1

A) Structure of the initial lead Stat3 inhibitor compound (C36) and structures of Stat3 inhibitor compounds identified through a similarity search (C29, C30 and C48). B) Dose-response effect of compound C48 on the DNA-binding activity of Stat1 and Stat3 homodimers, as well as Stat1/3 heterodimers in vitro, as assessed by EMSA. C48 and nuclear extract (NE) that contained activated STAT proteins were pre-incubated for 30 min prior to addition of DNA for 30 min. SS, Super-shifted STAT proteins. C) Mode of action of C48 on STAT DNA-binding. As a control, NE was incubated with DNA for 30 minutes (lanes 1-2), C48 and NE that contained activated STAT proteins were pre-incubated prior to addition of DNA (lanes 3-6), NE and DNA were pre-incubated prior to addition of C48 (lanes 7-10), or C48 and DNA were pre-incubated prior to addition of NE (lanes 11-14).