Figure 6. Effect of C48 on Stat3- and Stat1-mediated transcriptional activity.
A) Effect of C48 on Oncostatin M (OSM)-induced, Stat3-mediated expression of luciferase as a measure of transcription activity. Serum-starved HeLa-Stat3-Luc cells were pre-incubated with C48 1 h prior to stimulation with OSM. Luminescence was measured 8 h post stimulation. B) Effect of C48 on IFNγ-induced, Stat1-mediated expression of luciferase as a measure of transcription activity. Serum-starved HeLa-Stat1-Luc cells were pre-incubated with C48 1 h prior to stimulation with IFNγ. Luminescence was measured 8 h post stimulation. One representative result is shown (n=3, in triplicate).