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. Author manuscript; available in PMC: 2012 May 20.
Published in final edited form as: ACS Chem Biol. 2011 Feb 16;6(5):432–443. doi: 10.1021/cb100253e

Figure 7. Effect of C48 on cell viability and Stat3 signaling pathway proteins.

Figure 7

A-C) Effect of C48 on viability of the Stat3-pTyr705-positive cell lines MDA-MB-468 (A) and MDA-MB-231 (B) and the Stat3-pTyr705-negative cell line LNCaP (C). Cells were treated for 48 h with C48 or DMSO control and analyzed for Annexin V/PI staining. One representative result per cell line is shown (n=2, in triplicate). D-F) Western blot of total protein lysate from MDA-MB-468 cells treated with C48. D) Cells were treated for 48 h with C48 in the concentrations indicated prior to analysis for signaling proteins. E) Cells were treated for 4 h with 50 μM C48 prior to analysis of Jak2 and Src family phosphorylation. F) Cells were pre-treated for 30 min with Na3VO4 in the concentrations indicated, followed by addition of 50 μM C48 for 2 h. Cells were then analyzed for Stat3-pTyr705 levels.