Table 2.
Levels of Connexin-43 in Organoids in Preculture Groups as Assessed by Manual Counts of Immunostained Sections
| |
Number of Cx-43 dots/cell |
|
|---|---|---|
| Group | Nonstimulated | Biphasic |
| Pre FB | 1 ± 1 | 1 ± 1 |
| Pre EC | 2 ± 1a | 4 ± 2 |
| Pre FB and EC | 3 ± 2a | 5 ± 4 |
a
Indicates significantly smaller than CM biphasic as assessed by t-test. Within each stimulation regime, CM, Pre FB, Pre EC, Pre FB, and EC groups were compared by one-way ANOVA in conjunction with Dunn's test. Bracket indicates significant difference. For both nonstimulated and biphasic, the presence of Connexin-43 in CM groups (Table 1) was significantly higher than in the Pre FB and Pre EC groups. p < 0.05 considered significant. FB, fibroblast; EC, endothelial cell.