FIG. 3.

(A) Transplant harvested at 18 weeks, with 1000 μm lamellar spacing. Scaffold lamellae are widely spaced. Bone remains associated with scaffold. Magnification: 5×. (B) Histologic section at same location as in (A). Confirmation of the donor origin of the newly formed bone. In situ hybridization to ALU is localized to osteocytes in the new bone, and is absent in the peri-transplant tissues. Magnification: 5×. (C) Higher powered image of histologic section seen in (A). Magnification: 20×. (D) Histologic section at same location as in (C). Confirmation of the donor origin of the newly formed bone. In situ hybridization to ALU is localized to osteocytes in the new bone, and in BMSCs persisting from the time of transplantation. Magnification: 20× (b, bone; s, scaffold; arrows, alu-positive cells) Stain (A, C): hematoxylin and eosin; paraffin embedding following demineralization. Color images available online at www.liebertonline.com/tea