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. 2011 Mar 23;17(11-12):1605–1614. doi: 10.1089/ten.tea.2010.0447

FIG. 1.

FIG. 1.

Western blot and IHC of atrial appendage wall after 3 days of in vitro organ culture. Adding CoPP to the culture medium on day 0 induces HO-1 protein production. Inhibitor SnPP does not inhibit HO-1 protein production (but instead sterically inhibits HO-1 enzymatic activity). Culturing full-thickness atrial wall in the absence of CoPP results in low levels of endogenous HO-1 production, whereas no HO-1 is seen in freshly procured atrial wall that has not been cultured. On IHC, CoPP treatment results in widespread HO-1 expression (brown DAB staining) in cardiomyocytes, endothelial cells, endocardium, and epicardium. Atrial walls cultured without CoPP develop low levels (lighter staining) of HO-1 in cardiomyocytes, with patchy areas of darker staining in central cardiomyocytes. No HO-1 staining is seen in freshly procured, uncultured atrial tissue. Magnification bars=100 μm. IHC, immunohistochemistry; CoPP, cobalt protoporphyrin; SnPP, tin protoporphyrin; HO-1, heme oxygenase-1.