FIG. 3.

Examples of subcutaneous atrial grafts in vivo at 14 days postimplant, with IHC for ANP (brown DAB staining) to demonstrate cardiomyocyte content. Black dotted lines indicate graft margins, but are not those actually used to calculate graft volumes. (A) Graft pretreated with CoPP prior to implantation; (B) graft precultured prior to implantation without CoPP; (C) graft implanted with neither preculture nor CoPP treatment. Magnification bars=200 μM. Color images available online at www.liebertonline.com/tea