TABLE 1.
Affiliations of the 16S rDNA clones analyzed in this study
| OTUa | Sequence representative (EMBL accession no.) | No. of clones in library
|
Microorganism or clone with highest 16S rDNA sequence similarity
|
||||
|---|---|---|---|---|---|---|---|
| Aerobic basin | Anoxic basin | Digestor | Taxon | Accession no. | % Similarity | ||
| Planctomyces | |||||||
| 1 | 026B03_P_BA_P3 (BX294719) | 3 | 0 | 0 | Planctomyces sp. | AJ231189 | 92.2-92.8 |
| 2 | 032B06_P_BA_P3 (BX294869) | 1 | 0 | 0 | Planctomyces sp. | AB015527 | 90.5 |
| 3 | 026D07_P_BA_P3 (BX294724) | 3 | 3 | 0 | Planctomyces sp. | X81953 | 90.0-90.4 |
| 4 | 028F09_P_BN_P5 (BX294807) | 0 | 1 | 0 | Planctomyces sp. | AJ2311 | 89-91.9 |
| 5 | 031B01_P_BA_P3 (BX294828) | 1 | 0 | 0 | Planctomyces sp. | AB015527 | 89.6 |
| 6 | 032B01_P_BA_P3 (BX294867) | 1 | 0 | 0 | Planctomyces sp. | X81953 | 89.3 |
| 7 | 025A02_P_BA_P3 (BX294675) | 1 | 3 | 0 | Planctomyces sp. | X81953 | 89.2-90 |
| 8 | 027C06_P_BN_P5 (BX294754) | 0 | 1 | 0 | Planctomyces sp. | AJ231189 | 88.8 |
| 9 | 026A07_P_BA_P3 (BX294717) | 5 | 0 | 1 | Planctomyces sp. | AB015527 | 88.7-90.4 |
| 10 | 025B04_P_BA_P3 (BX294685) | 1 | 0 | 0 | Planctomyces maris | AJ231184 | 88.7 |
| 11 | 031F07_P_BA_P3 (BX294849) | 1 | 0 | 0 | Planctomyces brasiliensis | X85247 | 88.7 |
| 12 | 028B12_P_BN_P5 (BX294787) | 0 | 1 | 0 | Planctomyces brasiliensis | X85247 | 88.6 |
| 13 | 032C05_P_BA_P3 (BX294872) | 1 | 0 | 0 | Planctomyces sp. | X81953 | 88.5 |
| 14 | 032A02_P_BA_P3 (BX294862) | 1 | 0 | 0 | Planctomyces brasiliensis | X85247 | 88.4 |
| 15 | 025C03_P_BA_P3 (BX294689) | 1 | 0 | 0 | Planctomyces brasiliensis | X85247 | 88.1 |
| 16 | 028F03_P_BN_P5 (BX294803) | 0 | 1 | 0 | Planctomyces brasiliensis | X85247 | 88.1 |
| 17 | 028D04_P_BN_P5 (BX294795) | 1 | 1 | 0 | Planctomyces brasiliensis | X85247 | 88-88.4 |
| 18 | 026D10_P_BA_P3 (BX294726) | 1 | 0 | 0 | Planctomyces maris | AJ231184 | 87.8 |
| 19 | 025H12_P_BA_P3 (BX294716) | 1 | 0 | 0 | Planctomyces sp. | X81953 | 87.7 |
| 20 | 027B06_P_BN_P5 (BX294748) | 0 | 4 | 0 | Planctomyces brasiliensis | X85247 | 87.4-88.6 |
| 21 | 028B05_P_BN_P5 (BX294783) | 0 | 1 | 0 | Planctomyces brasiliensis | X85247 | 86.8 |
| 22 | 028G10_P_BN_P5 (BX294812) | 0 | 1 | 0 | Planctomyces sp. | AJ231189 | 86.8 |
| 23 | 030H03_P_DI_P15 (BX294821) | 0 | 0 | 1 | Planctomyces brasiliensis | X85247 | 86.8 |
| 24 | 056E05_P_DI_P58 (BX294905) | 0 | 0 | 1 | Planctomyces maris | AJ231184 | 84.2 |
| Total | 24 | 23 | 17 | 3 | |||
| Pirellula | |||||||
| 25 | 031B03_P_BA_P3 (BX294830) | 2 | 1 | 0 | Pirellula sp. | X81947 | 97.5-98.4 |
| 26 | 025E05_P_BA_P3 (BX294698) | 1 | 0 | 0 | Uncultured planctomycete | AJ290171 | 97.5 |
| 27 | 028A09_P_BN_P5 (BX294778) | 4 | 1 | 0 | Uncultured bacterium PHOS-HE25 | AF314429 | 97.3-99.5 |
| 28 | 055E08_P_DI_P58 (BX294901) | 0 | 1 | 1 | Uncultured planctomycete | AJ290171 | 97.0-97.3 |
| 29 | 056C02_P_DI_P58 (BX294903) | 1 | 1 | 1 | Uncultured planctomycete | AF271319 | 96.5-97.2 |
| 30 | 026G09_P_BA_P3 (BX294737) | 4 | 0 | 0 | Pirellula sp. | X81945 | 95.2-97.0 |
| 31 | 032B02_P_BA_P3 (BX294868) | 3 | 0 | 0 | Uncultured planctomycete | AJ290171 | 95.1-96.6 |
| 32 | 031C05_P_BA_P3 (BX294835) | 2 | 0 | 0 | Uncultured planctomycete | AF271331 | 95.0-95.5 |
| 33 | 027D05_P_BN_P5 (BX294759) | 0 | 3 | 0 | Uncultured planctomycete | AJ290171 | 94-95.3 |
| 34 | 027E08_P_BN_P5 (BX294767) | 1 | 1 | 1 | Uncultured sludge bacterium A17 | AF234760 | 94.4-95.6 |
| 35 | 028H06_P_BN_P5 (BX294815) | 1 | 2 | 2 | Uncultured planctomycete | AF271331 | 94.1-96.2 |
| 36 | 055D08_P_DI_P58 (BX294899) | 0 | 0 | 1 | Uncultured sludge bacterium A17 | AF234760 | 93.8 |
| 37 | 025G08_P_BA_P3 (BX294708) | 1 | 0 | 0 | Pirellula sp. | X81947 | 92.6 |
| 38 | 027A12_P_BN_P5 (BX294745) | 8 | 1 | 0 | Uncultured planctomycete | AJ290171 | 92.2-92.7 |
| 39 | 031F02_P_BA_P3 (BX294845) | 1 | 0 | 0 | Uncultured planctomycete | AF271319 | 92.2 |
| 40 | 028C11_P_BN_P5 (BX294792) | 0 | 1 | 0 | Uncultured sludge bacterium A4 | AF234736 | 92.0 |
| 41 | 025H10_P_BA_P3 (BX294715) | 1 | 0 | 0 | Pirellula staleyi | X81946 | 92.0 |
| 42 | 025A12_P_BA_P3 (BX294682) | 3 | 1 | 0 | Pirellula staleyi | X81946 | 91.8-93.3 |
| 43 | 055C03_P_DI_P58 (BX294898) | 4 | 1 | 1 | Uncultured planctomycete | AJ290171 | 91.6-91.8 |
| 44 | 027E04_P_BN_P5 (BX294764) | 0 | 1 | 0 | Pirellula staleyi | X81946 | 90.7 |
| 45 | 031A04_P_BA_P3 (BX294823) | 1 | 0 | 0 | Planctomycete str292 | P231182 | 90.2 |
| 46 | 028F08_P_BN_P5 (BX294806) | 0 | 1 | 0 | Uncultured planctomycete | AF271331 | 90.1 |
| 47 | 055H09_P_DI_P58 (BX294902) | 0 | 0 | 1 | Uncultured planctomycete | AF271331 | 90.0 |
| 48 | 027H04_P_BN_P5 (BX294772) | 0 | 1 | 0 | Pirellula staleyi | AJ231183 | 90.0 |
| 49 | 030F07_P_DI_P15 (BX294820) | 1 | 0 | 1 | Pirellula marina | X62912 | 89.6-90.8 |
| 50 | 025E11_P_BA_P3 (BX294700) | 2 | 0 | 0 | Pirellula sp. | X81945 | 89.4-89.6 |
| 51 | 025E01_P_BA_P3 (BX294696) | 1 | 0 | 0 | Uncultured planctomycete | AJ290171 | 89.4 |
| 52 | 025A04_P_BA_P3 (BX294677) | 1 | 0 | 0 | Pirellula staleyi | X81946 | 89.1 |
| 53 | 028D03_P_BN_P5 (BX294794) | 0 | 1 | 0 | Pirellula staleyi | AJ231183 | 88.9 |
| 54 | 026G04_P_BA_P3 (BX294733) | 4 | 0 | 0 | Uncultured Pirellula clone 55H12 | AF029076 | 87.8-88.1 |
| 55 | 025F07_P_BA_P3 (BX294704) | 1 | 0 | 0 | Pirellula sp. | X81945 | 87.8 |
| Total | 31 | 9 | 17 | 9 | |||
| Isosphaera | |||||||
| 56 | 032F01_P_BA_P3 (BX294885) | 1 | 0 | 0 | Isosphaera sp. | X81960 | 87.7 |
| Gemmata | |||||||
| 57 | 025D04_P_BA_P3 (BX294693) | 1 | 0 | 0 | Uncultured planctomycete | AJ290195 | 99/PICK> |
| 58 | 031A06_P_BA_P3 (BX294824) | 3 | 0 | 0 | Uncultured planctomycete | AJ290195 | 97.9-98.5 |
| 59 | 028F10_P_BN_P5 (BX294808) | 0 | 1 | 0 | Uncultured planctomycete | AJ290195 | 93.8 |
| 60 | 027A08_P_BN_P5 (BX294744) | 0 | 2 | 0 | Gemmata obscuriglobus | X81957 | 90.7 |
| 61 | 025D03_P_BA_P3 (BX294692) | 1 | 0 | 0 | Uncultured planctomycete | AF271341 | 89.9 |
| 62 | 028E08_P_BN_P5 (BX294799) | 0 | 1 | 0 | Gemmata obscuriglobus | X81957 | 87.4 |
| Total | 6 | 5 | 4 | 0 | |||
| Group I | |||||||
| 63 | 028D05_P_BN_P5 (BX294796) | 1 | 1 | 0 | Uncultured sludge bacterium H28 | AF234749 | 96.7-99.0 |
| 64 | 027A05_P_BN_P5 (BX294742) | 4 | 7 | 0 | Uncultured sludge bacterium H28 | AF234749 | 95.7-99.0 |
| 65 | 028E09_P_BN_P5 (BX294800) | 1 | 1 | 0 | Uncultured sludge bacterium H28 | AF234749 | 95.3 |
| 66 | 026G07_P_BA_P3 (BX294735) | 1 | 0 | 0 | Uncultured sludge bacterium H28 | AF234749 | 95.0 |
| 67 | 031G01_P_BA_P3 (BX294851) | 1 | 0 | 2 | Uncultured sludge bacterium H28 | AF234749 | 94.4-94.9 |
| 68 | 027H07_P_BN_P5 (BX294774) | 3 | 1 | 0 | Uncultured sludge bacterium H28 | AF234749 | 93.7-95.9 |
| 69 | 026F02_P_BA_P3 (BX294730) | 3 | 1 | 0 | Uncultured sludge bacterium H28 | AF234749 | 93.9-94.6 |
| 70 | 028F02_P_BN_P5 (BX294802) | 1 | 3 | 0 | Uncultured sludge bacterium H28 | AF234749 | 93.2-95.3 |
| 71 | 025A10_P_BA_P3 (BX294681) | 3 | 5 | 0 | Uncultured sludge bacterium H28 | AF234749 | 92.9-96.4 |
| 72 | 028H01_P_BN_P5 (BX294813) | 0 | 2 | 0 | Uncultured sludge bacterium H28 | AF234749 | 94.2-95.7 |
| 73 | 031E04_P_BA_P3 (BX294842) | 1 | 0 | 0 | Uncultured sludge bacterium H28 | AF234749 | 94.2 |
| 74 | 028A10_P_BN_P5 (BX294779) | 0 | 2 | 0 | Uncultured sludge bacterium H28 | AF234749 | 93.8-94.1 |
| 75 | 026B05_P_BA_P3 (BX294720) | 7 | 1 | 0 | Uncultured sludge bacterium H28 | AF234749 | 93.7-96.8 |
| 76 | 025C06_P_BA_P3 (BX294690) | 2 | 0 | 0 | Uncultured sludge bacterium H28 | AF234749 | 93.0-93.4 |
| 77 | 031H10_P_BA_P3 (BX294861) | 6 | 0 | 0 | Uncultured sludge bacterium H28 | AF234749 | 93.7-94.1 |
| 78 | 025E06_P_BA_P3 (BX294699) | 3 | 0 | 0 | Uncultured sludge bacterium H28 | AF234749 | 92.7-93.1 |
| 79 | 027E06_P_BN_P5 (BX294766) | 0 | 1 | 0 | Uncultured sludge bacterium H28 | AF234749 | 92.2 |
| 80 | 028B10_P_BN_P5 (BX294786) | 0 | 2 | 0 | Uncultured sludge bacterium H28 | AF234749 | 92.1-92.8 |
| 81 | 028H07_P_BN_P5 (BX294816) | 0 | 1 | 0 | Uncultured sludge bacterium H28 | AF234749 | 91.8 |
| 82 | 032F07_P_BA_P3 (BX294886) | 5 | 0 | 0 | Uncultured sludge bacterium H28 | AF234749 | 91.5-91.9 |
| 83 | 032G06_P_BA_P3 (BX294888) | 1 | 0 | 0 | Uncultured sludge bacterium H28 | AF234749 | 91.5 |
| 84 | 026E07_P_BA_P3 (BX294727) | 3 | 0 | 0 | Uncultured sludge bacterium H28 | AF234749 | 90.7-90.8 |
| 85 | 031G03_P_BA_P3 (BX294853) | 1 | 0 | 0 | Uncultured planctomycete | AJ290183 | 90.4 |
| 86 | 027D08_P_BN_P5 (BX294761) | 0 | 2 | 0 | Uncultured sludge bacterium H28 | AF234749 | 89.9-90.7 |
| 87 | 025G09_P_BA_P3 (BX294709) | 3 | 0 | 0 | Uncultured sludge bacterium H28 | AF234749 | 89.6-89.9 |
| 88 | 026A10_P_BA_P3 (BX294718) | 1 | 0 | 0 | Uncultured sludge bacterium H28 | AF234749 | 89.3 |
| Total | 26 | 51 | 30 | 2 | |||
| Group II | |||||||
| 89 | 027A06_P_BN_P5 (BX294743) | 0 | 1 | 0 | Uncultured planctomycete | AJ290183 | 89.1 |
| Group III | |||||||
| 90 | 027B07_P_BN_P5 (BX294749) | 0 | 1 | 0 | Uncultured planctomycete | AJ290171 | 89.2 |
| 91 | 028C07_P_BN_P5 (BX294790) | 0 | 1 | 0 | Uncultured sludge bacterium H28 | AF234749 | 88.5 |
| 92 | 032H11_P_BA_P3 (BX294894) | 1 | 0 | 0 | Planctomyces brasiliensis | X85247 | 85.3 |
| Total | 3 | 1 | 2 | 0 | |||
| Group IV | |||||||
| 93 | 055B12_P_DI_P58 (BX294897) | 0 | 0 | 1 | Pirellula sp. | X81945 | 84.9 |
| 94 | 029G09_P_DI_P15 (BX294818) | 0 | 0 | 1 | Uncultured planctomycete | X81945 | 83.9 |
| Total | 2 | 0 | 0 | 2 | |||
| Group V | |||||||
| 95 | 028H05_P_BN_P5 (BX294814) | 0 | 1 | 0 | Unidentified bacterium wb1_D18 | AF317785 | 81.1 |
| Total | 1 | 0 | 1 | 0 | |||
| Group VI | |||||||
| 96 | 025H04_P_BA_P3 (BX294712) | 1 | 0 | 0 | Uncultured Crater Lake bacteria | AF316773 | 91.1 |
| 97 | 028G08_P_BN_P5 (BX294811) | 0 | 1 | 0 | Unidentified bacteria wb 1-D18 | AF317785 | 90.4 |
| 98 | 031G08_P_BA_P3 (BX294856) | 1 | 0 | 0 | Uncultured Crater Lake bacteria | AF316773 | 90.3 |
| 99 | 031D04_P_BA_P3 (BX294837) | 1 | 0 | 0 | Uncultured Crater Lake bacteria | AF316773 | 90.2 |
| 100 | 025G12_P_BA_P3 (BX294710) | 1 | 0 | 0 | Uncultured Crater Lake bacteria | AF316773 | 90.0 |
| 101 | 032E06_P_BA_P3 (BX294883) | 1 | 0 | 0 | Uncultured Crater Lake bacteria | AF316773 | 89.4 |
| 102 | 025A05_P_BA_P3 (BX294678) | 1 | 0 | 0 | Uncultured Crater Lake bacteria | AF316773 | 88.6 |
| 103 | 027H11_P_BN_P5 (BX294775) | 0 | 1 | 0 | Unidentified bacteria wb1-D18 | AF317785 | 84.1 |
| 104 | 028B09_P_BN_P5 (BX294785) | 0 | 1 | 0 | Unidentified bacteria wb1-D18 | AF317785 | 83.3 |
| 105 | 025F10_P_BA_P3 (BX294705) | 1 | 0 | 0 | Unidentified bacteria wb1-D18 | AF317785 | 83.2 |
| 106 | 032D01_P_BA_P3 (BX294875) | 1 | 1 | 0 | Unidentified bacteria wb1-D18 | AF317785 | 83.0 |
| 107 | 032D06_P_BA_P3 (BX294877) | 1 | 0 | 0 | Unidentified bacteria wb1-D18 | AF317785 | 82.5 |
| 108 | 028E10_P_BN_P5 (BX294801) | 0 | 1 | 0 | Unidentified bacteria wb1-D18 | AF317785 | 82.4 |
| 109 | 031B05_P_BA_P3 (BX294831) | 1 | 0 | 0 | Unidentified bacteria wb1-D18 | AF317785 | 81.9 |
| 110 | 027B03_P_BN_P5 (BX294746) | 0 | 1 | 0 | Uncultured Crater Lake bacteria | AF316773 | 88.1 |
| Total | 15 | 10 | 6 | 0 | |||
| TOTAL | 110 | 140 | 78 | 16 | |||
a
Clones exhibiting levels of 16S rDNA sequence similarity of ≥97% were grouped in the same OTU.