TABLE 4.
Group-specific probe S-*-Plan-322-a-A-18 based on the 5′-3′ 16S rRNA sequence alignmenta
| Probe, target, environmental clone, or taxon (accession no.) | Sequenceb |
|---|---|
| Probe S-*-Plan-0322-a-A-18 | 5′ GTTCCGGTCGTGGGGGCC 3′ |
| Target | 5′ GGCCCCCACGACCGGAAC 3′ |
| 032F07_P_BA_P3 (BX294886) | 5′ ------------------ 3′ |
| Uncultured sludge bacterium H28 | 5′ ------------------ 3′ |
| (AF234749) | |
| Escherichia coli O157 (AB035920) | 5′ -A--AG--AC--U----- 3′ |
| Planctomyces maris (AJ231184) | 5′ ---------C--U--G-- 3′ |
| Pirellula marina (X62912) | 5′ -----GU-UC--U----- 3′ |
| Isosphaera pallida (X64372) | 5′ -----GG-AC--U----- 3′ |
| “Candidatus Kuenenia stuttgartiensis” | 5′ --U-GG--AC--U----- 3′ |
| (AF375995) | |
| “Candidatus Brocadia anammoxidans” | 5′ --U-GG--AC--U----- 3′ |
| (AF375994) | |
| Parachlamydia sp. (AF366365) | 5′ -A--G--AAC--U----- 3′ |
| Verrucomicrobium spinosum (X90515) | 5′ -A--AG--AC--U----- 3′ |
| Spirochaeta halophila (M88722) | 5′ -A--GG--AC--U----- 3′ |
| Paracoccus denitrificans (Y16930) | 5′ -AU-AG--AC--U----- 3′ |
a
The probe designation is in accordance with the Oligonucleotide Probe Database nomenclature (1).
b
Sequence for positions 305 to 322 (E. coli numbering [5]). Dashes indicate identity with the homologous nucleotides in the target sequence. There were at least three mismatches for all nontarget microorganisms.