Fig. 4.

Non-reference allele percentage distribution of detected variants in AAA patient pools. Non-reference allele (NRA) percentage distribution of detected variants in AAA pools is significantly deviating from the commonly observed pattern in non-pooled experiments with a clear peak for heterozygous mutations at 30–50% NRA and homozygous mutations close to 100% NRA. Dashed lines are indicative of a distribution of NRA% of novel and known variants from an exome sequencing of a healthy individual