Figure 3. Opposite I_KACh relaxation gating induced by Pilo.

A, macroscopic current recordings induced by 1 μm (left) and 3 μm Pilo (right) obtained with the same voltage protocol described in Fig. 2. B, relationship between the pre-pulse voltage and I/I_max ratio for currents induced by three Pilo concentrations (n = 4–6). Current magnitude measured at arrow was normalized to the maximum current elicited by pre-pulse to +60 mV. Opposite to ACh, the fraction of K_ACh channels activated by Pilo during the pre-pulse increased with stronger depolarizations. Note that Pilo-induced currents at −120 mV decayed slowly, while ACh-induced currents at −120 mV increased.