FIG. 3.
Analysis of L. lactis strains expressing VP2 fusions. Proteins from log-phase growing cultures were fractionated and analyzed by Western blotting with anti-VP2 (A) or anti-Nuc (B and C) antibodies. spUsp45, signal peptide from the Usp45 preprotein; cwaM6, cell wall anchor domain from the M6 preprotein; PP, protoplast; CW, cell wall; SN, supernatant; IBDV, purified IBDV; Nuc, S. aureus nuclease. The positions of pre-VP2 (pVP2) and VP2 and molecular mass standards (kilodaltons) are indicated.