TABLE 1.
Strain or plasmid | Relevant characteristicsa | Source or reference |
---|---|---|
Strains | ||
E. coli TG1 | 39 | |
L. lactis MG1363 | Plasmid-free strain | 16 |
Plasmids | ||
pHB-22R | Apr; A-segment cDNA of IBDV strain D6948 cloned into pGEM-T (Promega) | 5 |
pVE5506 | Emr; pIL252 derivative | 10 |
pVE5523 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-nucA-t1t2 | 10 |
pVE5524 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-nucA-cwaM6-t1t2 | 10 |
pVE5529 | Apr Emr pBS::pIL252::ttrpA::P59::nucA-t1t2 | 10 |
pVE5588 | Apr Emr; pBS::pIL252::ttrpA::P59::vp2-t1t2 | This work |
pVE5539 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-nucA-vp2-t1t2 | This work |
pVE5586 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-vp2-t1t2 | This work |
pVE5540 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-nucA-vp2-cwaM6-t1t2 | This work |
pVE5587 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-vp2-cwaM6-t1t2 | This work |
pVE5541 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-vp3-t1t2 | This work |
pVE5542 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-vp3-cwaM6-t1t2 | This work |
pVE5543 | Apr EMr; pBS::pIL252::ttrpA::P59::vp3-t1t2 | This work |
pBS refers to pBSII-KS+ (Stratagene), ttrpA refers to the trpA operon terminator of E. coli (8), P59 refers to a lactococcal promoter (48), spUsp45 refers to the signal peptide of Usp45 (47), nucA refers to the A form of staphylococcal nuclease (30), and t1t2 refers to the rrnB operon terminators of E. coli (32).