TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| E. coli TG1 | 39 | |
| L. lactis MG1363 | Plasmid-free strain | 16 |
| Plasmids | ||
| pHB-22R | Apr; A-segment cDNA of IBDV strain D6948 cloned into pGEM-T (Promega) | 5 |
| pVE5506 | Emr; pIL252 derivative | 10 |
| pVE5523 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-nucA-t1t2 | 10 |
| pVE5524 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-nucA-cwaM6-t1t2 | 10 |
| pVE5529 | Apr Emr pBS::pIL252::ttrpA::P59::nucA-t1t2 | 10 |
| pVE5588 | Apr Emr; pBS::pIL252::ttrpA::P59::vp2-t1t2 | This work |
| pVE5539 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-nucA-vp2-t1t2 | This work |
| pVE5586 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-vp2-t1t2 | This work |
| pVE5540 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-nucA-vp2-cwaM6-t1t2 | This work |
| pVE5587 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-vp2-cwaM6-t1t2 | This work |
| pVE5541 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-vp3-t1t2 | This work |
| pVE5542 | Apr Emr; pBS::pIL252::ttrpA::P59::spUsp45-vp3-cwaM6-t1t2 | This work |
| pVE5543 | Apr EMr; pBS::pIL252::ttrpA::P59::vp3-t1t2 | This work |
a
pBS refers to pBSII-KS+ (Stratagene), ttrpA refers to the trpA operon terminator of E. coli (8), P59 refers to a lactococcal promoter (48), spUsp45 refers to the signal peptide of Usp45 (47), nucA refers to the A form of staphylococcal nuclease (30), and t1t2 refers to the rrnB operon terminators of E. coli (32).