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Release of siRNA after proteolytic cleavage of PLL. (a) Multilayer sRAuNPs were incubated with or without trypsin in PBS and the concentration of released siRNA in supernatant were measured. (b) sR1(cy5)P AuNPs were incubated in RPMI 1640 medium containing serum with or without trypsin for 24 hr and the fluorescence was examined every hour.
