Table 3.
Effect of Hypoxia on Expression of Genes in Unactivated Hepatic Stellate Cells.
| Gene | Room Air | 0.5% Oxygen |
|---|---|---|
| Vascular Endothelial Growth Factor | 1.2 +/− 0.08 | 2.0 +/− 0.35 |
| Placental Growth Factor | 1.2 +/− 0.10 | 2.4 +/− 0.74 |
| Macrophage Migration Inhibitory Factor | 1.1 +/− 0.03 | 2.2 +/− 0.40a |
| Interleukin-13 Receptor α1 | 1.1 +/− 0.04 | 1.8 +/− 0.42 |
| Prolyl-4-hydroxylase-α1 | 1.1 +/− 0.09 | 2.4 +/− 0.90 |
| Prolyl-4-hydroxylase-α2 | 1.2 +/− 0.07 | 4.6 +/− 1.77a |
| Angiopoietin-like 4 | 1.2 +/− 0.08 | 1.1 +/− 0.10 |
| Ccr1 | 1.4 +/− 0.19 | 1.4 +/− 0.62 |
| Ccr5 | 1.3 +/− 0.24 | 0.9 +/− 0.15 |
| α2b adrenergic receptor | 0.9 +/− 0.11 | 2.9 +/− 1.44 |
Hepatic stellate cells were isolated from mice and allowed to attach for 18 hours. The cells were then exposed to room air or 0.5% oxygen for 18 hours. Levels of the genes indicated were quantified by real-time PCR.
a
Significantly different from hepatic stellate cells exposed to room air (p<0.05Data are expressed as means ± SEM; n = 4.