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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: J Immunol. 2010 Jun 25;185(3):1878–1886. doi: 10.4049/jimmunol.1001305

Figure 1. Cross-linking leukocyte PECAM reverses an anti-PECAM block in transmigration.

Figure 1

Human monocyte transmigration through unstimulated HUVEC monolayers grown on collagen gels. Monocytes were prelabelled on ice with blocking antibodies against PECAM (hec7, 177) and CD99 (hec2) at 20μg/ml, before being washed free of unbound antibody and added to HUVEC monolayers for 60 minutes. PECAM was activated by the addition of cross-linking secondary antibodies (50μg/ml) 30 minutes into the assay where indicated. Data represent mean ± SEM from 7 separate experiments. ** P<0.003, *** P<0.001.