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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: J Immunol. 2010 Jun 25;185(3):1878–1886. doi: 10.4049/jimmunol.1001305

Figure 2. Leukocyte PECAM becomes tyrosine phosphorylated after cross-linking.

Figure 2

(A) Human monocytes or (B) differentiated U937L cells, were stimulated by PECAM cross-linking for the indicated times and lysates were immunoprecipitated (IP) with anti-PECAM antibodies. Samples were resolved on SDS-PAGE gels and analysed by western blotting (IB) using anti-phosphotyrosine and PECAM antibodies. Band intensities were measured via densitometry, and the ratio of phosphorylated PECAM to total PECAM is shown below. (C) Differentiated U937L were left either untreated or pretreated with the Src kinase inhibitor PP2 (20 or 100μM, 30 minutes) before PECAM cross-linking for 3 minutes. Lysates were analysed by western blotting (performed as in A and B). Results shown are representative of 3 separate experiments.