Skip to main content
. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: J Immunol. 2010 Jun 25;185(3):1878–1886. doi: 10.4049/jimmunol.1001305

Figure 6. PECAM phosphorylation is reduced in DRMs.

Figure 6

U937L cells were left untreated or pretreated with 20μM MβCDX for 45min before undergoing PECAM cross- linking for 3 minutes. Soluble and insoluble fractions were immunoprecipitated (IP) with anti-PECAM antibodies and resolved on SDS-PAGE gels and analysed by western blotting (IB) using anti-phosphotyrosine and PECAM antibodies. Band intensities were measured via densitometry, and the ratio of phosphorylated PECAM to total PECAM is shown below from two separate experiments. Blots shown are representative of 3 separate experiments.