Figure 2. Association of Dyn1HL3 with the cell cortex occurs independently of plus end targeting.
(A – B) The percentage of cells that exhibit (A) plus end or (B) cortically associated Dyn1HL3-3YFP foci is plotted for wild-type (WT) and indicated null strains (n ≥ 105 cells). Stationary cortical or motile plus end foci were identified in two-color movies and scored accordingly. Error bars represent standard error of proportion. (C) Representative images of pac1Δ or bik1Δ cells expressing mCherry-Tub1 and Dyn1HL3-3YFP used for quantitation in panels (A) and (B). Closed arrowhead indicates cortical Dyn1HL3-3YFP foci. Each image is a maximum intensity projection of a 2-μm Z-stack of wide-field images.