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. Author manuscript; available in PMC: 2011 May 22.
Published in final edited form as: Mol Genet Metab. 2008 Nov 12;96(1):32–37. doi: 10.1016/j.ymgme.2008.10.005

Table 1.

Structure-function analysis of human G6PT

Ad-G6PT Microsomal G6P Uptake Activity Proteoliposomal G6P Uptake Activity Proteoliposomal Pi Uptake Activity
Mock 0.085 ± 0.003 0.31 ± 0.04 9.3 ± 0.3
Wild-type 0.332 ± 0.002 (100) 1.52 ± 0.01 (100) 112.7 ± 0.4 (100)
K240C 0.125 ± 0.006 (16.2) 0.50 ± 0.02 (15.7) 28.2 ± 0.5 18.3)
K240R 0.219 ± 0.008 (54.3) 0.90 ± 0.01 (48.8) 63.2 ± 1.2 (52.1)
H366D 0.131 ± 0.006 (18.6) 0.55 ± 0.03 (19.8) 29.7 ± 0.8 (19.7)
V369K 0.104 ± 0.003 (7.7) 0.48 ± 0.03 (14) 19.1 ± 0.3 (9.5)
H366D/V369K 0.105 ± 0.004 (8.1)

Microsomal G6P uptake activity (nmol/mg microsomal protein/3 min) was analyzed in microsomes isolated from COS-1 cells co-infected with Ad-G6Pase-α and Ad-G6PT or an Ad-G6PT mutant. Proteoliposomal G6P or Pi uptake activity (nmol/mg proteoliposomal protein/9 min) was analyzed in proteoliposomes reconstituted from detergent solubilized COS-1 microsomal membrane extracts expressing a wild-type or a mutant Ad-G6PT and were loaded with 50 mM Pi as described under Materials and methods. Results shown are from 3 independent experiments, each point determined in triplicate. Values represent mean ± SEM. Numbers in parentheses represent the percentage of wild-type transport activity.

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